- Author:
Jin-Shui HE
1
;
Yi-Qun HUANG
2
;
Jian-Ming WENG
3
;
Li-Yun XIAO
1
;
Kai-Zhi WENG
1
;
Xu-Dong MA
4
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Caspase 3; Cell Line, Tumor; Cell Proliferation; Heterocyclic Compounds, 3-Ring; Humans; Lymphoma, Mantle-Cell; RNA, Messenger; RNA, Small Interfering; Transfection; beta Catenin
- From: Journal of Experimental Hematology 2015;23(2):411-415
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of short hairpin RNA (shRNA) and XAV939, a specific inhibitor for β-catenin, on growth and apoptosis of mantle cell lymphoma(MCL) Jeko-1 cell line.
METHODSβ-catenin shRNA eukaryotic expression vector was transfected into Jeko-1 cells, the antiproliferative effect of shRNA on Jeko-1 cells was detected by RT-PCR and Western blot. The proliferation inhibitory rate of Jeko-1 cells treated by different doses of XAV939 was assayed by MTT method; the level of apoptosis of Jeko-1 cells was detected by flow cytometry; the expression levels of apoptosis-related protein BCL-2, BAX, CyclinD1, C-MYC and Caspase-3 in Jeko-1 cells were determined by Western blot.
RESULTSThe expression of β-catenin mRNA and growth of Jeko-1 cell line were inhibited by shRNA; after Jeko-1 cells treated with 0,2 and 8 µmol/L XAV939 for 48 hours, the cell proliferation rate decreased, while the cell apoptosis rate increased, the expressions of apoptosis-related protein BCL-2, CyclinD1 and C-MYC were down-regulated, on the contrary, the expression of BAX and caspase-3 were up-regulated.
CONCLUSIONThe specific inhibition of β-catenin can inhibit Jeko-1 cell proliferation and promote the cell apoptosis.