Isolation, idetification and anti-HIV-1 integrase activity of culturable endophytic fungi from Tibetan medicinal plant Phlomis younghusbandii Mukerjee.
- Author:
Da-Wei ZHANG
1
;
Ming-Ming ZHAO
;
Juan CHEN
;
Chao LI
;
Shun-Xing GUO
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Ascomycota;
enzymology;
isolation & purification;
Chaetomium;
enzymology;
isolation & purification;
Endophytes;
enzymology;
isolation & purification;
Escherichia coli;
enzymology;
HIV Integrase;
genetics;
metabolism;
HIV Integrase Inhibitors;
pharmacology;
Phlomis;
microbiology;
Phylogeny;
Plant Roots;
microbiology;
Plant Stems;
microbiology;
Plants, Medicinal;
microbiology;
Plasmids;
Recombinant Proteins;
genetics;
metabolism
- From:
Acta Pharmaceutica Sinica
2013;48(5):780-789
- CountryChina
- Language:Chinese
-
Abstract:
A total of 52 endophytic fungi were isolated from roots and stems of Tibetan medicinal plant Phlomis younghusbandii Mukerjee. These fungal isolates were molecularly identified based on ITS sequnces and 28S sequences distributed to 12 genera, including Phoma, Chaetosphaeronema, Fusarium and Leptosphaeria, etc. Among them, the dominant genus was Phoma. Extracts of all strains were evaluated for anti-HIV-1 integrase activity by using soluable integrase expressed in E. coli BL21 (DE3). The results showed that seven samples from five fungal endophytes PHY-24, PHY-38, PHY-40, PHY-51, PHY-53, which belonged to genus Chaetosphaeronema, inhibited strand transfer reaction catalyzed by HIV-1 integrase with IC50 values, of 6.60, 5.20, 2.86, 7.86, 4.47, 4.56 and 3.23 microg x mL(-1) respectively. In conclusion, the endophytic fungi of Phlomis younghusbandii Mukerjee are valuable for further screening anti-HIV-1 integrase agents.
