LC-MS quantification and pharmacokinetics of the multi-constituents of Huangqin Tang in rat plasma after different single oral doses.
- Author:
Tao LI
1
;
Yi-Wei WANG
;
Yan-Li WANG
;
Ri-Xin LIANG
;
Dong ZHANG
;
Hui-Hui ZHANG
;
Li CHEN
;
Zhong-Ming ZHOU
;
Wei-Peng YANG
Author Information
1. Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
- Publication Type:Journal Article
- MeSH:
Administration, Oral;
Animals;
Chromatography, Liquid;
Dose-Response Relationship, Drug;
Drugs, Chinese Herbal;
administration & dosage;
chemistry;
Flavanones;
blood;
pharmacokinetics;
Flavonoids;
blood;
pharmacokinetics;
Glucosides;
blood;
pharmacokinetics;
Glycyrrhetinic Acid;
blood;
pharmacokinetics;
Glycyrrhizic Acid;
blood;
pharmacokinetics;
Male;
Monoterpenes;
blood;
pharmacokinetics;
Pentacyclic Triterpenes;
blood;
pharmacokinetics;
Rats;
Rats, Wistar;
Spectrometry, Mass, Electrospray Ionization
- From:
Acta Pharmaceutica Sinica
2013;48(6):917-924
- CountryChina
- Language:Chinese
-
Abstract:
The current study aims to investigate the pharmacokinetic properties of Huangqin Tang on different oral doses. An LC-MS method for simultaneous determination of flavonoids and terpenoids in rat plasma was developed and validated. Plasma samples were treated with hydrochloric acid (containing 1% ascorbic acid), precipitated with acetonitrile, separated on a Zorbax SB-C18 column, detected by single quadruple mass spectrometry with an electrospray ionization interface, and quantified using selected ion monitoring mode. All pharmacokinetic parameters were processed by non-compartmental analysis using WinNonlin software. The results of specificity, linearity, intra-day and inter-day precisions, accuracy, and stability for LC-MS assay were suitable for the quantification of paeoniflorin, baicalin, wogonoside, baicalein, wogonin, oroxylin A, glycyrrhizic acid and glycyrrhetinic acid in rat plasma. The concentration-time profiles of baicalin, wogonoside, baicalein, wogonin, oroxylin A and glycyrrhizic acid showed double-peak phenomenon after Huangqin Tang was orally administered at 40 g x kg(-1) dose; all eight constituents in rat plasma showed good dose-exposure relationship within the dosage of 10-40 g x kg(-1); although plasma concentrations were different, the flavonoids with the same backbone showed the similar fate in the body with the corresponding dosage. In conclusion, the LC-MS assay was successfully applied for the pharmacokinetic study of multi-constituents of Huangqin Tang with different doses. Additionally, these constituents demonstrated good pharmacokinetic properties in the body.
