Tanshinone IIA protects against triptolide-induced liver injury via Nrf2/ARE activation.
- Author:
Cui-wen GUAN
1
;
Jing JIN
1
;
Jia LI
1
;
Zhong-xiang ZHAO
2
;
Zhi-ying HUANG
1
Author Information
1. School of Pharmaceutical Sciences, Sun Yat-sen University, Guangzhou 510006, China.
2. School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antioxidant Response Elements;
drug effects;
Chemical and Drug Induced Liver Injury;
metabolism;
pathology;
Diterpenes;
toxicity;
Diterpenes, Abietane;
pharmacology;
Drugs, Chinese Herbal;
pharmacology;
Epoxy Compounds;
toxicity;
Glutamate-Cysteine Ligase;
genetics;
metabolism;
Heme Oxygenase-1;
genetics;
metabolism;
Liver;
metabolism;
pathology;
Male;
Membrane Proteins;
genetics;
metabolism;
Mice;
Mice, Inbred C57BL;
NAD(P)H Dehydrogenase (Quinone);
genetics;
metabolism;
NF-E2-Related Factor 2;
metabolism;
Phenanthrenes;
toxicity;
RNA, Messenger;
metabolism;
Signal Transduction;
drug effects
- From:
Acta Pharmaceutica Sinica
2013;48(9):1397-1402
- CountryChina
- Language:Chinese
-
Abstract:
The aim of this study is to investigate the protection effect of tanshinone IIA (Tan) against triptolide (TP)-induced liver injury and the mechanisms involved. Acute liver injury was induced by intraperitoneal injection of TP (1 mg x kg(-1)) in mice. The activities of AST, ALT and LDH in serum and the levels of GSH, GST, GSH-PX, SOD, CAT and MDA in liver tissue were detected. The histopathological changes of liver tissues were observed after HE staining. Nrf2 translocation in liver tissue was detected by Western blotting, and real-time PCR was used to measure the expression levels of GCLC, NQO1 and HO-1 mRNA. The results showed that pretreatment with Tan significantly prevented the TP induced liver injury as indicated by reducing the activities of AST, ALT and LDH (P < 0.01). Tan pretreatment also prevented TP-induced oxidative stress in the mice liver by inhibiting MDA and restoring the levels of GSH, GST, SOD and CAT (P < 0.05). Parallel to these changes, pretreatment with Tan could attenuate histopathologic changes induced by TP. Furthermore, the results indicated that Tan pretreatment caused nuclear accumulation of Nrf2 as well as induction of mRNA expression of antioxidant response element (ARE)-driven genes such as GCLC, NQO1 and HO-1. These results indicated that Tan could protect against TP-induced acute liver injury via the activation of Nrf2/ARE pathway.
