An experimental study on segmental defects reconstruction of canine mandible with allogenic bone marrow mesenchymal stem cells combined with lyophilized bone
10.3760/cma.j.issn.1002-0098.2015.12.004
- VernacularTitle:同种异体骨髓间充质干细胞复合冻干骨修复下颌骨节段性缺损的实验研究
- Author:
Hong WANG
1
;
Caixia JING
;
Xinying TAN
;
Jinchao LUO
;
Changkui LIU
;
Min HU
Author Information
1. 解放军总医院口腔颌面外科
- Keywords:
Myeloid progenitor cells;
Mesenchymal stem cells;
Mandible;
Reconstruction
- From:
Chinese Journal of Stomatology
2015;50(12):720-724
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of segmental defects reconstruction of canine mandible with allogenenic bone marrow mesenchymal stem cells (BMSC) combined with lyophilized bone.Methods A 30 mm segmental defect was created on the left mandibles of beagles.Beagles were randomly divided into three groups.Allogeneic bone marrow mesenchymal stem cells with lyophilized bone were used for segmental defects reconstruction in group A.Autologous bone marrow mesenchymal stem cells with freeze-dried bone were used for segmental defects reconstruction in group B.The defects of group C were repaired with lyophilized bone only.Every three beagles were sacrificed 4, 12, 24, and 48 weeks after surgery respectively.The reconstruction effect was evaluated by CT and histopathological examination.Results CT examination showed that new bones formed in group A and group B 12 weeks after surgery, but not in group C.The form of the reconstructed mandibles in the three groups recovered in 48 weeks.The small pores on the bone graft were filled with new bones in group A and group B.In group C, the pores were still evident.Histopathological examination showed that bone trabecula between allogeneic bone and autogenous bone was completely joined in group A and group B.A large number of fibrous tissue appeared around the implanted bone and new bones were formed.In group C, the lyophilized bone resorption was still not obvious, the new bone formation was significantly slower than the other two groups.There was no difference between group A and group B.Conclusions Both allogeneic bone marrow mesenchymal stem cells and autologous mesenchymal stem cells could accelerate the bone formation.
