Distribution of mast cells and its role in the pathogenesis of Hirschsprung disease.
- Author:
Heng ZHENG
1
;
Yu-min CHEN
;
Ming-fa WEI
;
Zhi-yi GUO
;
Shi-yu ZHAO
;
Ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Child; Child, Preschool; Female; Hirschsprung Disease; metabolism; pathology; Humans; Infant; Intestinal Mucosa; pathology; Male; Mast Cells; cytology; metabolism; pathology; Tryptases; metabolism
- From: Chinese Journal of Gastrointestinal Surgery 2009;12(5):507-510
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the distribution of mast cells (MC) in colon tissue of Hirschsprung disease (HD) and explore the role of mast cells in the pathogenesis of HD.
METHODSForty-one cases of HD (male 23, female 18), age from 2 months to 15 years, and eight age-matched normal cases were enrolled in this study. The distribution of MC in all layers of colon was examined by immunohistochemistry with mouse antihuman mast cell tryptase monoclonal antibody.
RESULTSThe count of MC in all layers of colon aganglionic segments of HD was significantly higher as compared with colon ganglionic segments of HD and normal controls (21.47+/-3.59 vs 3.18+/-0.87, 2.75+/-0.51). The average optical density values(A) of MC in aganglionic and ganglionic segments significantly decreased as compared to normal control (0.38+/-0.10,0.31+/-0.11 vs 0.51+/-0.08).
CONCLUSIONMast cells may play an important role in the pathogenesis of HD.
