Effects of duodenal-jejunal bypass and sleeve gastrectomy on the expression of liver glucokinase in diabetic rats.
- Author:
Dong-lei ZHOU
1
;
Ding-yu ZHANG
;
Xu-guang HU
;
Dan-lei CHEN
;
Zhong-wei KE
;
Kai YIN
;
Ji-hui LI
;
Cheng-zhu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Glucose; analysis; Diabetes Mellitus, Experimental; metabolism; surgery; Digestive System Surgical Procedures; methods; Duodenum; surgery; Gastrectomy; Glucokinase; metabolism; Jejunum; surgery; Liver; metabolism; Male; Rats; Rats, Inbred Strains; Rats, Sprague-Dawley
- From: Chinese Journal of Gastrointestinal Surgery 2009;12(6):558-561
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of duodenal-jejunal bypass(DJB) and sleeve gastrectomy(SG) on the expression of liver glucokinase(GCK) in diabetic rats.
METHODSAnimal models of Goto-Kakizaki rats and Sprague-Dawley rats were established by DJB and SG. Results of fasting glycemia and insulin were compared. Liver tissue was harvested 8 weeks postoperatively.Quantitative real-time PCR and Western blot were used to detect liver GCK mRNA and protein expression after operation.
RESULTSFasting plasma glucose levels of DJB group and SG group in GK rats were markedly declined 3 day and 1, 2, 4, 6, 8 weeks postoperatively(all P <0.01), while Sham group only dropped 3 day and 1 week postoperatively, and there were no significant differences 2 weeks postoperatively(P >0.05). Fasting plasma glucose levels of each group in SD rats did not change after operation. In GK rats, GCK mRNA level (1.45 +/-0.29) and protein expression (494.25 +/-30.25) after DJB were higher than Sham group (1.05 +/-0.19 and 409.13 +/-26.86) and control group (1.04 +/-0.17 and 404.75 +/-30.90). GCK mRNA level and protein expression after SG were 0.65 +/-0.25 and 345.25 +/-28.13 respectively, which were significantly lower than those in control group(all P <0.01). All the groups in SD rats experienced similar GCK expression change.
CONCLUSIONBoth DJB and SG can decrease the plasma glucose levels of GK rats, while there are different effects on the expression of liver GCK.
