Experimental research of the inhibition of vascular endothelial growth factor C expression in gastric cancer by targeting RNA interference.
- Author:
Guo-Ting CHEN
1
;
Xian-Ping NIU
;
Qi LI
;
Sheng-Chao JI
;
Qing-Hui HAN
;
Yang-Zhou LIU
;
Xia LI
;
Hui ZHANG
;
Duan CAI
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line, Tumor; Genetic Vectors; Humans; Plasmids; RNA Interference; RNA, Small Interfering; Stomach Neoplasms; genetics; Transfection; Vascular Endothelial Growth Factor C; genetics
- From: Chinese Journal of Gastrointestinal Surgery 2010;13(1):64-67
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the plasmid expression vector pSIH1-H1-copGFP for RNA interference against vascular endothelial growth factor C (VEGF-C) and to evaluate its effect on the expression of VEGF-C mRNA in gastric cancer cells after transfection.
METHODSThree siRNAs of genome sequence of VEGF-C gene were retrieved from GenBank and one negative chain was used as control. Four siRNAs were cloned into plasmid pSIH1-H1-copGFP,which were then transfected into gastric cancer cells (SGC7901). The expression of VEGF-C mRNA was analyzed by RT-PCR.
RESULTSThe recombinant plasmid of pSIH1-H1-copGFP specific for VEGF-C was confirmed by gene sequencing analysis. The target sequence obtained was completely consistent with the design. Transfection efficiency of the three siRNAs ranged from 60% to 70%. After transfection, the expression of VEGF-C mRNA in SGC7901 cells was significantly inhibited. Inhibition rates of VEGF-C mRNA expression were 35.4%, 33.8% and 81.5% in the three siRNA plasmid vectors, respectively.
CONCLUSIONThe siRNA expression plasmid vector against VEGF-C mRNA is successfully constructed, and RNAi may be a useful technique to inhibit the lymphangiogenesis of gastric cancer.
