TLR4 signals are involved in multiple myeloma cell proliferation and apoptosis.
- Author:
Han-Ying BAO
1
;
Li-Juan WANG
;
Yang YANG
;
Zhen CAI
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; physiology; Cell Line, Tumor; Cell Proliferation; Humans; Multiple Myeloma; immunology; metabolism; pathology; Signal Transduction; Toll-Like Receptor 4; metabolism
- From: Journal of Zhejiang University. Medical sciences 2009;38(5):465-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the TLR4 signaling in multiple myeloma cell proliferation and apoptosis.
METHODSTLR4 gene transcription and protein expression were detected by RT-PCR and PE flow cytometry staining; the cells proliferation were detected by MTT assay; doxorubicin-induced apoptosis of cells was detected by AnnexinV-PI double staining flow cytometry.
RESULTTLR4 gene transcription and protein expression was detected in the myeloma cell lines. Under the lipopolysaccharides (LPS) stimulation, MM1-s cells TLR4 positive proliferation significantly increased (P<0.05), but not found in U266 cells TLR4 negative; MM1-s cells showed the resistance to doxorubisin-induced apoptosis, but LPS did not protect doxorubicin-induced U266 cell apoptosis.
CONCLUSIONTLR4 signaling may play an important role both in multiple myeloma proliferation and survival.
