An in vitro hepatic steatosis cell model for study of non-alcoholic fatty liver disease.
- Author:
Jiang LIU
1
;
You-Ming LI
;
Shao-Hua CHEN
;
Wei-Ping LI
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; pharmacology; Fatty Acids, Nonesterified; pharmacology; Fatty Liver; Hep G2 Cells; Humans; Models, Biological; Non-alcoholic Fatty Liver Disease; Oleic Acid; pharmacology; Palmitates; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2009;38(6):626-629
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an in vitro cell model to investigate hepatic steatosis of non-alcoholic fatty liver disease.
METHODSHepG2 cells cultured in MEM containing 10 % fetal bovine serum were divided into control group and model group. At 7 0%-80 % confluency, HepG2 cells in model group were exposed to a long-chain mixture of free fatty acids (oleate and palmitate) for 24 h, cells in control group were subject to fresh medium. Lipid droplets were observed with oil red O stain and electron microscope, triglyceride and malonaldehyde were detected by respective assay kits.
RESULTA large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However,malonaldehyde did not increase significantly compared with control group.
CONCLUSIONA large number of lipid droplet were detected in model HepG2 cells; the level of triglyceride increased. However, malonaldehyde did not increase significantly compared with control group.
