Down-regulation of Nrf2 enhances the cytotoxicity of Oxaliplatin to H460 cells.
- Author:
Ai XIN
1
;
Hua-yu REN
;
Xiu-wen TANG
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents; pharmacology; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; pathology; Cell Line, Tumor; Cell Proliferation; drug effects; Down-Regulation; Drug Resistance, Neoplasm; genetics; Humans; Lung Neoplasms; genetics; metabolism; pathology; NF-E2-Related Factor 2; genetics; metabolism; Organoplatinum Compounds; pharmacology; Transfection
- From: Journal of Zhejiang University. Medical sciences 2010;39(1):11-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a stable H460 cell line with Nrf2 down-regulation to study the role of Nrf2 in Oxaliplatin resistance.
METHODSNrf2 down-regulated H460 cell line was obtained by transfecting cells with pRS-hNrf2 and followed by screening for Nrf2 expression by Western blotting. The cell lines were further characterized by analysing cellular GSH levels and cytotoxicity to anti-cancer drugs with MTS.
RESULTNrf2 down-regulated H460 cell lines were established successfully. Cellular GSH level reduced significantly in the H460-C9 and H460-C13 compared to control cells (P(C9)= 0.00249, P(C13)= 0.03944). Down-regulation of Nrf2 in H460 sensitized the anti-cancer effect of Oxaliplatin and Doxorubicin.
CONCLUSIONNrf2 plays an important role in drug resistance. Down regulation of Nrf2 in H460 cell enhances cytotoxicity of Oxaliplatin.
