Effect of DRB/alpha-Amanitin on localization of Nrf2 in A549 cells.
- Author:
Li-yan QU
1
;
Yan-ling JIANG
;
Xiu-wen TANG
Author Information
- Publication Type:Journal Article
- MeSH: 20-Hydroxysteroid Dehydrogenases; genetics; metabolism; Alpha-Amanitin; pharmacology; Carcinoma, Non-Small-Cell Lung; genetics; metabolism; pathology; Cell Line, Tumor; Dichlororibofuranosylbenzimidazole; pharmacology; Heme Oxygenase-1; genetics; metabolism; Humans; Lung Neoplasms; metabolism; pathology; NF-E2-Related Factor 2; genetics; metabolism; Nucleic Acid Synthesis Inhibitors; pharmacology
- From: Journal of Zhejiang University. Medical sciences 2010;39(1):24-29
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of transcriptional inhibitors 5, 6-dichloro-1-b-D-ribofuranosylbenzimidazole (DRB) and alpha-Amanitin on the localization of Nrf2 in the nucleus.
METHODSA549 cells were treated with DRB (50 mg/L) or alpha-Amanitin (2.5 mg/L)for 1 h and 6 h in serum-free medium, respectively. The expressions of Nrf2, HO-1, NQO1 and AKR1C were detected by Western blotting analysis. The localization of Nrf2 was determined by laser scanning confocal microscopy after cells were treated with either DRB or agr:-Amanitin for 1 h.
RESULTSThe expressions of Nrf2 and Nrf2-ARE gene batteries HO-1, AKR1C and NQO1 were decreased after 6 h treated with either DRB or alpha-Amanitin. The expression of SC35 was up-regulated but RNA Pol II was down-regulated; Y12 and NPC did not significantly change. The localization of Nrf2 in the cell nucleus did not change significantly.
CONCLUSIONDRB and alpha-Amanitin can down-regulate the expression of Nrf2 and its targeting proteins HO-1, AKR1C and NQO1, but may have no effect on the localization of Nrf2.
