Inhibitive effects of chimeric oncolytic adenovirus SG235 on leukemia cells in vitro.
- Author:
Chun-mei YANG
1
;
Hui LIU
;
Xiu-di YANG
;
Jie JIN
;
Wen-bin QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Apoptosis; Cell Line, Tumor; Cell Proliferation; Genetic Vectors; Humans; Leukemia; genetics; metabolism; pathology; Membrane Cofactor Protein; metabolism; Oncolytic Viruses; Transfection
- From: Journal of Zhejiang University. Medical sciences 2010;39(3):226-230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the inhibitive effects of chimeric oncolytic adenovirus SG235 on leukemia cells in vitro.
METHODSThe ability of SG235 to infect leukemia cells and the expression of CD46 on blasts from the patient with leukemia were detected by flow cytometry (FACS). The cytotoxicity of the virus was evaluated by MTT assay. Apoptosis induced by SG235 was detected with Annexin-V/PI staining and TUNEL assay followed by FACS analysis.
RESULTThe majority of leukemia cells from the patient with acute leukemia was CD46-positive. GFP-positive cells were 45.1%, 35.7%, 54.2%, 37.0%, 30.1%, %67.1, 17.2% and 33.1% in Mutz-1, Kasumi-1, K562, HL60, Molt- 4, RPMI8226, L428, and Jurkat cell lines treated with SG235-EGFP vector at MOI (multiplicity of infection) of 50 for 48 h.SG235 treatment resulted in marked growth inhibition and apoptosis of Kassumi-1 cells, and also significantly inhibited expression of p-Akt.
CONCLUSIONThe chimeric oncolytic adenovirus SG235 can infect leukemia cell effectively and results in the growth inhibition and apoptosis of Kasumi-1 cells in vitro.
