Formaldehyde inhalation may damage olfactory bulb and hippocampus in rats.
- Author:
Yi-qiao LI
1
;
Hao-hao CHEN
;
Yi-fei YIN
;
Fei HAN
;
Xue-song YE
;
Shu-cai LING
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Formaldehyde; toxicity; Glutamic Acid; metabolism; Hippocampus; drug effects; metabolism; pathology; Inhalation Exposure; Learning; drug effects; Neurons; drug effects; metabolism; pathology; Nitric Oxide Synthase; metabolism; Olfactory Bulb; drug effects; metabolism; pathology; Rats; Rats, Sprague-Dawley; gamma-Aminobutyric Acid; metabolism
- From: Journal of Zhejiang University. Medical sciences 2010;39(3):272-277
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of formaldehyde inhalation on the morphological damage, and Glu, GABA and NOS contents in olfactory bulb and hippocampus of rats.
METHODSTwenty SD rats were equally divided into two groups: rats in the control group inhaled fresh air, while the animals in experimental group were exposed to the air containing formaldehyde (12.5 mg/m(3), 4 h/d) for 7 days. Then rats were sacrificed and frozen sections of olfactory bulb and hippocampus were prepared. The morphological changes were examined and the Glu, GABA and NOS contents were detected using Nissl-staining, immunohistochemistry and Western blot, respectively.
RESULTCompared with the control group, there was a significant confusion and shrink of neuron morphology in experimental group, the number and staining intensity of Glu and NOS positive cells and protein contents were reduced. The protein expression of GABA was also decreased in the formaldehyde group.
CONCLUSIONFormaldehyde inhalation can cause a severe morphological damage of olfactory bulb and hippocampus in SD rats,which may further impair memory and learning ability through the reduction of Glu, GABA and NOS expression.
