Preparation of a novel targeted MR contrast agent Gd-DTPA-Granzyme B monoclonal antibody.
- Author:
Yi XIE
1
;
Guang-yi LIU
;
Fei HAN
;
Hong JIANG
;
You-ying MAO
;
Hui-ping WANG
;
Jie ZHAO
;
Juan JIN
;
Jiang-hua CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Antibodies, Monoclonal; chemistry; Cells, Cultured; Contrast Media; chemistry; toxicity; Gadolinium DTPA; chemistry; Granzymes; immunology; Magnetic Resonance Imaging; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
- From: Journal of Zhejiang University. Medical sciences 2010;39(3):290-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo prepare a novel MRI targeted contrast agent Gd-DTPA-Granzyme B monoclonal antibody (mAb) and to test its reaction conditions.
METHODSThe Granzyme B mAb was coupled with DTPA,and then conjugated with Gd. The Gd-DTPA antibody was characterized using MALDI-TOF-MS. Cytotoxicity test was performed with MTT assay, and immune activation was examined with immunohistochemistry.
RESULTMALDI-TOF-MS demonstrated that the molecular weight shifted from granzyme B mAb (133986) to Gd-DTPA-GB mAb (139736), which indicated the conjugation of the antibody with Gd-DTPA. The molar ratio of Gd per IgG molecule was about 20. MTT assay showed that Gd, DTPA, Gd-DTPA and Gd-DTPA-GB mAb groups did not make an impact on cell viability, and there were no significant differences among 4 groups (P>0.05). Immunohistochemistry results showed that compared with the positive control group the targeted contrast agent had a high immune activity.
CONCLUSIONThe novel contrast agent Gd-DTPA-Granzyme B mAb prepared in this study keeps a good immune activity and has no significant cytotoxicity.
