2-dimensional gel electrophoresis on protein patterns from suspension cultures of Taxus cuspidate induced by SA.
- Author:
Jian-Jun QIAO
1
;
Hong ZHAO
;
Zhi-Qiang GE
;
Ying-Jin YUAN
;
An-Ping ZENG
Author Information
1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China.
- Publication Type:Journal Article
- MeSH:
Cell Culture Techniques;
methods;
DNA, Plant;
genetics;
Electrophoresis, Agar Gel;
Electrophoresis, Gel, Two-Dimensional;
methods;
Gene Expression Regulation, Plant;
drug effects;
Plant Proteins;
genetics;
metabolism;
Salicylates;
pharmacology;
Taxus;
genetics;
metabolism
- From:
Chinese Journal of Biotechnology
2003;19(1):92-96
- CountryChina
- Language:Chinese
-
Abstract:
The permeability of cell membrane was enhanced by exogenous SA in the culture of Taxus cuspidata. Nuclei condense and fragments were observed in some cells by using fluorescent microscope, and the degraded chromosomal DNA was observed by using agarose gel electrophoresis. The changes in soluble proteins of the suspension cultures of Taxus cuspidata induced by salicylic acids were analyzed by using two-dimensional polyacrylamide gel electrophoresis. Comparing with the control, seven new protein spots were found and six protein spots were not found in the cultures grown with SA at 48h. The results obtained showed that SA could induce the expression of some special proteins that might be related with the action of SA in the suspension cultures of Taxus cuspidata.
