Expression of phytase gene in Bombyx mori.
- Author:
Wen-Bing WANG
1
;
Bin YAO
;
Qing-Li XIAO
;
Ping JI
;
Sheng-Peng WANG
;
Jia-Lu HE
;
Xiang-Fu WU
Author Information
1. Key Laboratory of Silkworm Biotechnology, Ministry of Agriculture, Zhenjiang 212018, China.
- Publication Type:Journal Article
- MeSH:
6-Phytase;
genetics;
metabolism;
Animals;
Aspergillus niger;
enzymology;
genetics;
Blotting, Southern;
Bombyx;
genetics;
metabolism;
Electrophoresis, Polyacrylamide Gel;
Fungal Proteins;
genetics;
metabolism;
Genetic Vectors;
genetics;
Hydrogen-Ion Concentration
- From:
Chinese Journal of Biotechnology
2003;19(1):112-115
- CountryChina
- Language:Chinese
-
Abstract:
Phytase gene of Aspergillus niger 963 was cloned into baculovirus transfer vector. DNA of the recombinant vector was co-transfected with Bm-BacPAK6 DNA into BmN cells, and recombinant virus was selected by plaque assays. The recombinant virus was identified by Dot blot and Southern-blot with the specific probe for phytase gene. Phytase gene was expressed in silkworm larvae and pupae. The expression product was 1.43 g/L haemolymph for silkworm larvae and 1.90 g/L haemolymph for pupae, respectively. The enzymic characteristicses of phytase expressed in baculovirus-expression system were studied in this paper.
