Using single primer PCR to amplify the orf25 gene fragment related to plant male-sterility in Salicornia europaea.
- Author:
Jin-Yao LI
1
;
Fu-Chun ZHANG
;
Ji MA
;
Wen-Juan SHAN
;
Bin WANG
Author Information
1. College of Life science and Technology, Xinjiang University, Urumuqi 830046, China.
- Publication Type:Journal Article
- MeSH:
Base Sequence;
Chenopodiaceae;
genetics;
DNA Primers;
genetics;
Molecular Sequence Data;
Plant Infertility;
genetics;
physiology;
Plant Proteins;
chemistry;
genetics;
Reverse Transcriptase Polymerase Chain Reaction;
methods;
Sequence Homology, Nucleic Acid
- From:
Chinese Journal of Biotechnology
2003;19(1):120-123
- CountryChina
- Language:Chinese
-
Abstract:
The gene orf25 encodes functional protein that may play an important role in plant fertility control in nature. To clone the orf25 from Salicornia europaea Xinjiang into a T-vector, a single designed primer was used to amplify 1.7kb cDNA fragment with RT-PCR. Sequence analysis reveals that the cloned fragment contains entire orf25 coding region with 98%, 95%, 92% and 88% identity to that of orf25 from Beta vulgaris, Nicotiana, wheat and maize mitochondrion, respectively. This analysis suggests that orf25 gene is highly conserved in terms of evolution in plant; and it also suggests that wild plant Salicornia europaea contain a male-sterility gene similar to crops that is of great importance in improvement of the breed of crop.
