Intracellular Antibody Fragment Against Hepatitis B Virus X Protein Does Not Inhibit Viral Replication.
10.3349/ymj.2006.47.5.721
- Author:
Young Hee JIN
1
;
Seung Ho HONG
;
Kyongmin KIM
;
Ho Joon SHIN
;
Sun PARK
Author Information
1. Department of Microbiology, Ajou University School of Medicine, Suwon, Korea. sinsun@ajou.ac.kr
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
Antibodies;
viral;
hepatitis B virus;
trans-activators;
virus replication
- MeSH:
Virus Replication/*drug effects;
Trans-Activators/*antagonists & inhibitors/immunology;
Immunoglobulin Variable Region/genetics/metabolism/*pharmacology;
Hepatitis B virus/*drug effects/physiology;
Hepatitis B e Antigens/metabolism;
Cell Line
- From:Yonsei Medical Journal
2006;47(5):721-728
- CountryRepublic of Korea
- Language:English
-
Abstract:
Replication of the hepatitis B virus is suppressed by deficiency of the X protein. Although several molecules that block cellular targets of X protein reduce the production of hepatitis B virus progeny, the effect of a specific inhibitor of X protein on viral replication has not been investigated. To block X protein specifically, we adopted an intracellular expression approach using H7 single chain variable fragment (H7scFv), an antibody fragment against X protein. We previously demonstrated that cytoplasmic expression of H7scFv inhibits X protein-induced tumorigenicity and transactivation. In this study, intracellular H7scFv expression inhibits reporter gene transactivation but not viral replication determined by endogenous hepatitis B virus polymerase activity assay and real-time PCR. Our findings imply that intracellular expression of antibody fragment against X protein may not be an alternative therapeutic modality for inhibition of hepatitis B virus replication.
