Recovery and purification of monoclonal antibody producted by continuous perfusion culture using expended bed adsorption.
- Author:
Xiao-Ling YU
1
;
Li MI
;
Xi-Ying YAO
;
Zhi-Nan CHEN
Author Information
1. The Fourth military medical university, National Engineering Research Center, Xi'an 710032, China.
- Publication Type:Journal Article
- MeSH:
Adsorption;
Antibodies, Monoclonal;
biosynthesis;
isolation & purification;
Bioreactors;
Cell Culture Techniques;
methods;
Chromatography;
methods;
Hybridomas;
cytology;
metabolism
- From:
Chinese Journal of Biotechnology
2004;20(2):227-232
- CountryChina
- Language:Chinese
-
Abstract:
Monoclonal antibody producted by continuous perfusion culture was recovered and purified by expended bed adsorption chromatography. A packed bed column XK16/20 was used for method scouting with Streamline SP adsorbent. Two expended bed columns Streamline-25 and -50 were used for method optimization and pilot scale experiment, respectively. The recovery yield of monoclonal antibody was above 90% in a 5 - 7 fold enhanced purity and 10 fold increased concentration. According to the different concentration of monoclonal antibody in cell culture broth, about 18 - 50L fluid can be treated in a single cycle. MAb purification from lab scale (400mg per cycle) to a small pilot scale (2g per cycle) has been achieved. Compared with packed bed adsorption, the preparation cycle was half shortened, and the cost of production and the complexity of process were decreased markedly. It has been proven that a purification process based on expended bed adsorption technique is simple, efficient and economical.
