The mechanism of Stat3 nuclear import.
- Author:
Zhong-De YE
1
;
Bei-Fen SHEN
;
Lun SONG
Author Information
1. Institute of Basic Medical Sciences, Academy of Military Medical Sciences, Beijing 100850, China.
- Publication Type:Journal Article
- MeSH:
Active Transport, Cell Nucleus;
Cell Nucleus;
metabolism;
Nuclear Localization Signals;
Plasmids;
metabolism;
STAT3 Transcription Factor;
metabolism
- From:
Chinese Journal of Biotechnology
2004;20(2):299-301
- CountryChina
- Language:Chinese
-
Abstract:
In order to investigate the mechanism of stat3 nuclear import. positioned a characterized NLS of the SV40 large T antigen into Stat3-GFP, Dstat3-GFP respectively between the C-terminus of Stat3 and the N-terminus of GFP to create Stat3-NLS-GFP and Dstat3-NLS-GFP. With NLS-GFP as the positive control, Expression of the Stat3-NLS-GFP without IL-6 stimulation and Stat3-GFP with IL-6 stimulation resulted in a predominantly nuclear localization in 293T cell. Expression of Stat3-GFP and Dstat3-NLS-GFP without IL-6 stimulation resulted in predominantly cytoplasm localization in 293T cell. The results suggest that latent Stat3 is not anchored in the cytoplasm, and that nuclear localization in response to IL-6 is facilitated by gain of an NLS function.
