Extraction and HPLC analysis of alkaloids in goldenseal.
- Author:
Holly A WEBER
1
;
Maureen JOSEPH
Author Information
1. Midwest Research Institute 425 Volker Blvd Kansas City, MO 64110, USA.
- Publication Type:Journal Article
- MeSH:
Benzylisoquinolines;
analysis;
isolation & purification;
Berberine;
analysis;
isolation & purification;
Chromatography, High Pressure Liquid;
Hydrastis;
chemistry;
Plant Roots;
chemistry
- From:
Chinese Journal of Biotechnology
2004;20(2):306-308
- CountryChina
- Language:Chinese
-
Abstract:
An ambient extraction of goldenseal root powder followed by HPLC analysis of the alkaloids on a Zorbax Rapid Resolution Eclipse XDB-C18 column provides an accurate method for the determination of key alkaloids in goldenseal, including berberine and hydrastine. The extraction and HPLC analysis can be applied to several other alkaloids, including canadine, hydrastinine, and palmatine, and may be applicable to other berberine-containing plant roots. The Rapid Resolution Eclipse XDB-C18 column is used for an isocratic separation with high resolution of all componentsin under 15 minutes.
