Screening of serum protein biomarkers by surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry in patients with mycosis fungoides.
- Author:
Jie LIU
1
;
Yan-Hua ZHENG
;
Jian-Zhong ZHANG
;
Bao-Xi WANG
Author Information
- Publication Type:Journal Article
- MeSH: Biomarkers, Tumor; blood; Blood Proteins; analysis; Humans; Mycosis Fungoides; blood; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; methods
- From: Acta Academiae Medicinae Sinicae 2009;31(1):13-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo screen serum biomarkers in patients with mycosis fungoides (MF) using surface-enhanced laser desorption and ionization with time-of-flight detection mass spectrometry (SELDI-TOF-MS) technique.
METHODSSerum was analyzed from 14 patients with MF and 17 controls using CM10 Protein-chip to capture serum proteins, followed by Biomarker Wizard software analysis.
RESULTSIn all specimens, about 131 protein peaks could be detected when the relative molecular weight ranged from 0 to 50 000. When comparing the protein fingerprint between these two groups, 14 differentially expressed protein peaks were found. By searching SWISS-PRO database, we found 7 670Da peaks accord with C-C motif chemokine 22.
CONCLUSIONSELDI-TOF-MS technique can be used for screening serum protein biomarkers in patients with MF.
