Detection and identification of mycobacterial gene in skin lesions and lymph nodes in patients with sarcoidosis.
- Author:
Xiao-Lan DING
1
;
Lin CAI
;
Jian-Zhong ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; DNA, Bacterial; analysis; Female; Humans; Lymph Nodes; microbiology; Male; Middle Aged; Mycobacterium; genetics; isolation & purification; Mycobacterium Infections; microbiology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Sarcoidosis; microbiology; Skin; microbiology; Young Adult
- From: Acta Academiae Medicinae Sinicae 2009;31(1):20-23
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the possible role of mycobacteria in the pathogenesis of sarcoidosis.
METHODSWe used nested polymerase chain reaction-restriction fragment length polymorphism (nPCR-RFLP) and gene sequencing method to examine 31 formalin-fixed, paraffin-embedded specimens (including 19 skin lesions and 12 lymph nodes ) obtained from patients with sarcoidosis, 14 normal skin specimens, and 3 cutaneous tuberculosis specimens.
RESULTSThe 65kD mycobacterial heat shock protein gene was found in 4 out of 19 (21.1%) skin specimens of sarcoidosis. The mycobacteria included M. tuberculosis (n = 1), M. chelonei (n = 2), and M. gordonae (n = 1). Mycobacterial DNA was negative in 12 lymph node specimens and 14 normal skin specimens. M. tuberculosis gene was detected in all 3 specimens of cutaneous tuberculosis.
CONCLUSIONMycobacteria may play a role in the pathogenesis of cutaneous sarcoidosis.
