Effect of neuronal differentiation induced by nerve growth factor on the tolerance-dosage of ultraviolet radiation of PC12 cells.
- Author:
Dan-Hui LI
1
;
Hao-Xiang XU
;
Ping SONG
;
Ya-Gang ZUO
;
Xiang-Hong YAN
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Differentiation; Cell Transformation, Neoplastic; drug effects; radiation effects; Dose-Response Relationship, Radiation; Nerve Growth Factor; pharmacology; Neurons; cytology; PC12 Cells; Rats; Ultraviolet Rays
- From: Acta Academiae Medicinae Sinicae 2009;31(1):24-26
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the effect of neuronal differentiation induced by nerve growth factor (NGF) on the tolerance-dosage of ultraviolet radiation of PC12 Cells.
METHODSNeuron-differentiated PC12 cells and untreated PC12 cells were exposed to different ultraviolet radiation dosage of 10, 30, 60, 80, 100, and 200 mJ/cm2. Cell survival rates were determined by MTT assay.
RESULTSNeuron-differentiated PC12 cells had increased tolerance dose to ultraviolet radiation with noticeable apoptosis at the radiation dose of 100 mJ/cm2 in contrast to 30 mJ/cm2 for normal PC12 cells.
CONCLUSIONNeuronal differentiation exerts the effect of increasing the tolerance dose of PC12 cells to ultraviolet radiation.
