Gene expression profile of isoniazid liver-injured rat using cDNA microarray.
- Author:
Yan LIAO
1
;
Shuang-qing PENG
;
Li-shi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antitubercular Agents; toxicity; Chemical and Drug Induced Liver Injury; etiology; metabolism; Disease Models, Animal; Gene Expression Profiling; Isoniazid; toxicity; Liver; drug effects; metabolism; pathology; Male; Oligonucleotide Array Sequence Analysis; Rats; Rats, Wistar; Transcriptome
- From: Acta Academiae Medicinae Sinicae 2009;31(3):308-314
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the changes of gene expression profile of rat liver tissue by cDNA microarrays.
METHODSTwenty Wistar rats in control group (n = 10) and isoniazid (INH) group (n = 10) were orally administrated with normal saline and 400 mg/kg INH for 14 days, respectively. The differentially expressed genes significantly correlated with liver injury were screened and analyzed. The mechanisms of liver injury caused by INH were specifically analyzed at level of gene expression based on the biological functions of those differentially expressed genes.
RESULTSThirty-seven differentially expressed genes were found in the rats administrated with INH. Among them, 25 genes were up-regulated, while the other 12 genes were down-regulated. These differentially expressed genes were functionally related to the changes of CYP450-related genes, fatty acid metabolism, and protein metabolism.
CONCLUSIONINH can cause the remarkable changes of the gene expression profiles in rat liver cells, which is important for further elucidating the mechanisms of liver injury caused by INH.
