Method for drainage of lymph fluid and determining the change of active materials in lymph fluid after rat ischemia-reperfusion injury.
- Author:
Xue-Feng CHEN
1
;
Gui-Zhen HE
;
Liang-Guang DONG
;
Hong SHU
;
Xiu-Rong WANG
;
Feng-Yan GAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Disease Models, Animal; Drainage; methods; Endotoxins; metabolism; HMGB1 Protein; metabolism; Interleukin-6; metabolism; Intestines; blood supply; metabolism; Lymph; metabolism; Male; Rats; Rats, Sprague-Dawley; Reperfusion Injury; metabolism; Tumor Necrosis Factor-alpha; metabolism
- From: Acta Academiae Medicinae Sinicae 2009;31(3):322-325
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo set up a method for the drainage of lymph fluid and explore the change of active materials in lymph fluid and serum after rat ischemia-reperfusion injury.
METHODSThe method of the drainage of lymph fluid was well established. Sixteen healthy male rats of SPF grade were selected and randomly divided into 2 groups: intestinal ischemia-reperfusion + drainage group (I/R + drainage group) and drainage group. All the rats were subjected to superior mesenteric artery occlusion for 60 minutes, followed by 120 minutes of reperfusion. We compared the change of high mobility group box-1 (HMGB1) protein, endotoxin tumor necrosis factor alpha (TNF-alpha), interleukin (IL) -1 beta, IL-6, and soluble vascular cell adhesion molecular-1 (sICAM-1) by draining lymph fluid and collecting serum in 2 groups.
RESULTSThe drainage of lymph fluid was successfully performed. The HMGB1, endotoxin, and cytokines in serum and lymph fluid were significantly higher in ischemia-reperfusion group than in drainage group (P < 0. 05).
CONCLUSIONSThe method for drainage of lymph fluid is simple and feasible. Endotoxin, HMGB1, and some cytokines in serum and lymph fluid may mediate the ischemia-reperfusion injury.
