The Effects of Ethyl Pyruvate on Lipopolysaccharide-induced Acute Lung Injury.
10.4046/trd.2006.61.4.374
- Author:
Seung Hyeun LEE
1
;
Dae Wui YOON
;
Jin Yong JUNG
;
Kyung Joo LEE
;
Se Joong KIM
;
Eun Joo LEE
;
Eun Hae KANG
;
Ki Hwan JUNG
;
Sung Yong LEE
;
Sang Yeub LEE
;
Je Hyeong KIM
;
Chol SHIN
;
Jae Jeong SHIM
;
Kwang Ho IN
;
Se Hwa YOO
;
Kyung Ho KANG
Author Information
1. Department of Internal Medicine, College of Medicine, Korea University, Seoul, Korea. kkhchest@korea.ac.kr
- Publication Type:In Vitro ; Original Article
- Keywords:
Acute lung injury;
Lipopolysaccharide;
Ethyl pyruvate
- MeSH:
Acute Lung Injury*;
Animals;
Bronchoalveolar Lavage Fluid;
Cytokines;
Humans;
Interleukin-6;
Lung;
Male;
Mice;
NF-kappa B;
Peroxidase;
Pyruvic Acid*;
Tumor Necrosis Factor-alpha
- From:Tuberculosis and Respiratory Diseases
2006;61(4):374-383
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: Ethyl pyruvate (EP) is a derivative of pyruvate that has recently been identified by both various in vitro and in vivo studies to have antioxidant and anti-inflammatory effects. The aim of this study was to determine the effect of EP on lipopolysaccharide (LPS)-induced acute lung injury (ALI). METHODS: 5 weeks old, male BALB/c mice were used. ALI was induced by an intratracheal instillation of LPS 0.5mg/Kg/50microliter of saline. The mice were divided into the control, LPS, EP+LPS, and LPS+EP groups. In the control group, balanced salt solution was injected intraperitoneally 30 minutes before or 9 hours after the intratracheal instillation of saline. In the LPS group, a balanced salt solution was also injected intraperitoneally 30 minutes before or 9 hours after instillation the LPS. In the EP+LPS group, 40mg/Kg of EP was injected 30 minutes before LPS instillation. In the LPS+EP group, 40mg/Kg of EP was injected 9 hours after LPS instillation. The TNF-alpha and IL-6 concentrations in the bronchoalveolar lavage fluid (BALF), and that of NF-KappaB in the lung tissue were measured in the control, LPS and EP+LPS groups at 6 hours after instillation of saline or LPS, and the ALI score and myeloperoxidase (MPO) activity were measured in all four groups 24 and 48 hours after LPS instillation, respectively. RESULTS: The TNF-alpha and IL-6 concentrations were significantly lower in the EP+LPS group than in the LPS group (p<0.05). The changes in the concentration of these inflammatory cytokines were strongly correlated with that of NF-kappaB (p<0.01). The ALI scores were significantly lower in the EP+LPS and LPS+EP groups compared with the LPS group (p<0.05). In the EP+LPS group, the MPO activity was significantly lower than the LPS group (p=0.019). CONCLUSION: EP, either administered before or after LPS instillation, has protective effects against the pathogenesis of LPS-induced ALI. EP has potential theurapeutic effects on LPS-induced ALI.
