Differentially expressed proteins between normal ovaries and ovarian cancer tissues screened by the protein chips.
- Author:
Fei CHEN
1
;
Jia-Xin YANG
;
Ming WU
;
Keng SHEN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Humans; Middle Aged; Neoplasms, Glandular and Epithelial; metabolism; Ovarian Neoplasms; metabolism; Ovary; metabolism; Protein Array Analysis; Proteomics
- From: Acta Academiae Medicinae Sinicae 2009;31(3):378-380
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the differentially expressed proteins between normal ovaries and ovarian cancer tissues using the protein chips.
METHODSTissues of 11 epithelial ovarian cancer (EOC) and 11 matched normal ovaries were labeled with cy3 and cy5 fluorescent dyes and then were hybridized with 512 monoclonal protein antibody chips. The internally normalized ratio (INR) was calculated according to the intensity of fluorescence of each protein spots. The value of INR > 2.0 or < 0.7 was considered as the cut-value to filtrate the differentially expressed proteins between tissues of EOC and normal ovaries.
RESULTSThirty one differentially expressed proteins were found between tissues of EOC and normal ovaries, in which 17 up-regulated and 14 down-regulated proteins involved in the transcription, proliferation, signal conduction, and apoptosis of cells.
CONCLUSIONAntibody chips can effectively screen the differentially expressed proteins between normal ovaries and ovarian cancer tissues.
