Establishment and evaluation of nitrate reductase combined with mycobacteriophage assay.
- Author:
Ai-Ying XING
1
;
Zhong-Quan LIU
;
Hong-Yan JIA
;
Shu-Xiang GU
;
Zong-De ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Bacterial Agents; pharmacology; Biological Assay; methods; Humans; Microbial Sensitivity Tests; methods; Mycobacteriophages; physiology; Mycobacterium tuberculosis; drug effects; Nitrate Reductase; metabolism; Rifampin; pharmacology; Sensitivity and Specificity
- From: Acta Academiae Medicinae Sinicae 2009;31(4):413-416
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a rapid, inexpensive, and simple drug susceptibility test (DST) for Mycobacterium tuberculosis (M. tb) and evaluate its feasibility.
METHODWe used nitrate reductase combined with mycobacteriophage assay (PhaB-NRA) to test 49 clinical M. tb isolates of, and the results were compared with those of PhaB-NRA and traditional absolute concentration method.
RESULTSThe sensitivity, specificity, and accuracy of PhaB-NRA for rifampicin were 89.1%, 91.67%, and 89.8%; on the contrary, those of isonicotinyl hydrazide were 86.21%, 90.0%, and 87.8%, respectively. The coincidence between PhaB-NRA and traditional assay were 0.746 for rifampicin and 0.750 for isonicotinyl hydrazide.
CONCLUSIONSPhaB-NRA is an inexpensive, rapid, and simple DST method. It is a promising rapid screening technique for DST of M. tb.
