OBJECTIVETo expound the therapeutic method based on the layers of "skin, vessel, muscle, tendon and bone" and its clinical values.

METHODSSystematically explore the literature relative to treatments based on the layers in The Yellow Emperor's Internal Classic, cite, analyze and summarize contents of nine types of needling and needling methods, etc., relative to treatments based on the layers.

RESULTSThe principle and therapeutic methods based on the layers of "skin, vessel, muscle, tendon and bone" are expounded and it is hold that this therapeutic method is one of key theories of ancient acupuncture and moxibustion therapy. The theory of channels and collaterals and treatment based on the layers are two important aspects in acupuncture and moxibustion treatment. Combination of the two aspects can increase therapeutic effect of acupuncture and moxibustion.

CONCLUSIONThe treatment based on layers of "skin, vessel, muscle, tendon and bone" is of important significance for recognition of acupuncture values in The Yellow Emperor's Internal Classic, a new establishment of diagnosis and examination, syndrome differentiation, treatment system of acupuncture and moxibustion independent of drug prescription, enriching acupuncture and moxibustion therapy, increasing the therapeutic effect of acupuncture and moxibustion, and extending the range of acupuncture and moxibustion treatment.