Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals.

Faridah Yusof; Atheer Awad Mehde; Wesen Adel Mehdi; Raha Ahmed Raus; Hamid Ghazali; Azlina Abd Rahman

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Effects of Nephrolithiasis on Serum DNase (Deoxyribonuclease I and II) Activity and E3 SUMO-Protein Ligase NSE2 (NSMCE2) in Malaysian Individuals.

Author: Faridah YUSOF ¹ ; Atheer Awad MEHDE ² ; Wesen Adel MEHDI ³ ; Raha Ahmed RAUS ¹ ; Hamid GHAZALI ⁴ ; Azlina Abd RAHMAN ⁵
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1. Department of Biotechnology Engineering, College of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia.
2. Department of Biotechnology Engineering, College of Engineering, International Islamic University Malaysia, Kuala Lumpur 50728, Malaysia
3. Department of Chemistry, College of Sciences for Women, University of Baghdad 10071, Iraq.
4. Department of Urology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia.
5. Department of Pathology, Hospital Tengku Ampuan Afzan, Kuantan 25200, Malaysia.
Publication Type:Journal Article
Keywords: DNase I; DNase II; E3 SUMO-protein ligase NSE2; NSMCE2; Nephrolithiasis
MeSH: Adult; Blood Proteins; analysis; Case-Control Studies; Creatinine; blood; Deoxyribonuclease I; blood; Endodeoxyribonucleases; blood; Hemoglobins; analysis; Humans; Ligases; blood; Malaysia; Middle Aged; Nephrolithiasis; blood; enzymology; Urea; blood
From: Biomedical and Environmental Sciences 2015;28(9):660-665
CountryChina
Language:English
Abstract:
OBJECTIVENephrolithiasis is one of the most common disorders of the urinary tract. The aim of this study was to examine a possible relationship between DNase I/II activity and E3 SUMO-protein ligase NSE2 in the sera of nephrolithiasis patients to evaluate the possibility of a new biomarker for evaluating kidney damage.
METHODSSixty nephrolithiasis patients and 50 control patients were enrolled in a case-control study. Their blood urea, creatinine, protein levels and DNase I/II activity levels were measured by spectrometry. Serum NSMCE2 levels were measured by ELISA. Blood was collected from patients of the government health clinics in Kuantan-Pahang and fulfilled the inclusion criteria.
RESULTSThe result indicated that mean levels of sera NSMCE2 have a significantly increase (P<0.01) in patients compared to control group. Compared with control subjects, activities and specific activities of serum DNase I and II were significantly elevated in nephrolithiasis patients (P$lt;0.01).
CONCLUSIONThis study suggests that an increase in serum concentrations of DNase I/II and E3 SUMO-protein ligase NSE2 level can be used as indicators for the diagnosis of kidney injury in patients with nephrolithiasis.