Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China.

Yan Ming LI; Xun Liang Tong; Hong Tao XU; Yang JU; Meng Cai; Chen Wang

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Prevalence and Antimicrobial Susceptibility of Mycobacterium abscessus in a General Hospital, China.

Author: Yan Ming LI ^{1
,

2} ; Xun Liang TONG ³ ; Hong Tao XU ⁴ ; Yang JU ⁵ ; Meng CAI ⁶ ; Chen WANG ⁷
Author Information

1. Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing 100730, China
2. Department of Hospital Infection Control and Management, Beijing Hospital, Beijing 100730, China.
3. Department of Geriatrics, Beijing Hospital, Beijing 100730, China.
4. Department of Laboratory Medicine, Beijing Hospital, Beijing 100730, China.
5. Department of Respiratory and Critical Care Medicine, Beijing Hospital, Beijing 100730, China.
6. Department of Hospital Infection Control and Management, Beijing Hospital, Beijing 100730, China.
7. Department of Respiratory Medicine, Chinese-Japanese Friendship Hospital, Beijing 100029, China.
Publication Type:Journal Article
Keywords: China; Mycobacterium abscessus; Nontuberculous mycobacteria
MeSH: China; Drug Resistance, Bacterial; Humans; Microbial Sensitivity Tests; Nontuberculous Mycobacteria
From: Biomedical and Environmental Sciences 2016;29(2):85-90
CountryChina
Language:English
Abstract:
OBJECTIVETo gain greater insight into the prevalence drug resistant profiles of M. abscessus from a general hospital in Beijing, China.
METHODSPartial gene sequencing of 16S, hsp65, and rpoB were used to distinguish the species of NTM isolates. All strains identified as M. abscessus were further enrolled in the drug susceptibility testing by using broth microdilution method.
RESULTSWe found that M. avium complex was the most frequent NTM organism, accounting for 54.1% (33/61) of all isolates. Behind MAC, the second most common organisms were M. abscessus (22 out of 61, 36.1%). Average rates of resistance were 4.5% for AMK, 9.1% for LZD, and 13.6% for CLA, respectively. In contrast, resistance to LEV (17/22, 77.3%), IMI (9/22, 40.9%), and SMX (10/22, 45.5%) was noted in more than 40% of M. abscessus isolates. DNA sequencing revealed that all the CLA-resistant isolates harbored nucleotide substitutions in position 2058 (1/3, 33.3%) or 2059 (2/3, 66.7%) of 23S rRNA.
CONCLUSIONIn conclusion, our data demonstrated that M. intracellulare and M. abscessus were the most common NTM species in the general hospital of Beijing. CLA, AMK, LZD showed promising activity, where as LEV, IMI, and SMX exhibited poor activity against M. abscessus in vitro.