Gene Knockdown in Human Rhinovirus 1B Using 2'-OMe-modified siRNAs Results in the Reactivation of the Interferon Response.

Guang Cheng Xie; Qing Zhang; Li Li Pang; Dan Di LI; Miao Jin; Hui Ying LI; Zi Qian XU; Xiang Yu Kong; Hong Wang; Shan LU; Zhao Jun Duan

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Gene Knockdown in Human Rhinovirus 1B Using 2'-OMe-modified siRNAs Results in the Reactivation of the Interferon Response.

Author: Guang Cheng XIE ¹ ; Qing ZHANG ¹ ; Li Li PANG ² ; Dan Di LI ¹ ; Miao JIN ¹ ; Hui Ying LI ¹ ; Zi Qian XU ¹ ; Xiang Yu KONG ¹ ; Hong WANG ¹ ; Shan LU ³ ; Zhao Jun DUAN ¹
Author Information

1. Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
2. Key Laboratory of Medical Virology and Viral Disease, Ministry of Health of the People's Republic of China, National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
3. National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China.
Publication Type:Journal Article
MeSH: Gene Knockdown Techniques; HeLa Cells; Humans; Interferons; physiology; RNA, Small Interfering; Rhinovirus; Virus Replication
From: Biomedical and Environmental Sciences 2016;29(2):137-142
CountryChina
Language:English
Abstract: The aim of this study was to investigate the knockdown efficiency of 2'-O-methylated (2'-OMe)-modified small interfering RNAs (siRNAs) on human rhinovirus 1B (HRV1B) replication and the interferon response. Thus, 24 2'-OMe-modified siRNAs were designed to target HRV1B. The RNA levels of HRV1B, Toll-like receptor 3, melanoma differentiation-associated gene 5, retinoic acid inducible gene-I, and interferons were determined in HRV1B-infected HeLa and BEAS-2B epithelial cells transfected with 2'-OMe-modified siRNAs. The results revealed that all 2'-OMe-modified siRNAs interfered with the replication of HRV1B in a cell-specific and transfection efficiency-dependent manner. Viral activation of Toll-like receptor 3, melanoma differentiation-associated gene 5, retinoic acid inducible gene-I, and the interferon response was detected. In conclusion, the 2'-OMe-modified siRNAs used in this study could interfere with HRV1B replication, possibly leading to the reactivation of the interferon response.