Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis.

Fatma Guesmi; Hamida Beghalem; Amit K Tyagi; Manel Ben Ali; Ramla Ben Mouhoub; Houda Bellamine; Ahmed Landoulsi

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Prevention of H2O2 Induced Oxidative Damages of Rat Testis by Thymus algeriensis.

Author: Fatma GUESMI ^{1
,

2} ; Hamida BEGHALEM ³ ; Amit K TYAGI ⁴ ; Manel Ben ALI ⁵ ; Ramla Ben MOUHOUB ³ ; Houda BELLAMINE ⁶ ; Ahmed LANDOULSI ³
Author Information

1. Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, Zarzouma, Bizerte 7021, Tunisia
2. Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston TX 77030, USA.
3. Laboratory of Biochemistry and Molecular Biology, Faculty of Sciences of Bizerte, Zarzouma, Bizerte 7021, Tunisia.
4. Cytokine Research Laboratory, Department of Experimental Therapeutics, The University of Texas M. D. Anderson Cancer Center, 1515 Holcombe Boulevard, Houston TX 77030, USA.
5. Laboratory of Biomonitoring of the Environment (LR01/ES14), Faculty of Sciences of Bizerte, Zarzouma, Bizerte 7021, Tunisia.
6. Service of Anatomo- Pathology of Menzel Bourguiba, Bizerte 7050, Tunisia.
Publication Type:Journal Article
Keywords: Antioxidants; Hydrogen peroxide; Infertility; TEO; Testis
MeSH: Animals; Hydrogen Peroxide; toxicity; Male; Oxidative Stress; Plant Extracts; pharmacology; Rats; Rats, Wistar; Testis; drug effects; Thymus Plant; chemistry
From: Biomedical and Environmental Sciences 2016;29(4):275-285
CountryChina
Language:English
Abstract:
OBJECTIVEWe evaluate the effects of Thymus algeriensis (TEO) against hydrogen peroxide (H2O2) toxicity on body and testis weight, testis sperm count, testis lipid peroxidation, and antioxidant enzyme activities in rats.
METHODSRats were treated with low (LD) and high dose (HD) of H2O2 (0.1 and 1 mmol/L) in the presence or absence of TEO (150 mg/kg).
RESULTSThe results exhibited a significant decrease in body weight and testis weight, in total sperm number decrease (P<0.05), sperm motility and percentage of sperm viability, leading to complete arrest, in sperm flagellar beat frequency by the gavage of 1 mmol/L H2O2 compared to controls. The administration of H2O2 resulted in a significant reduction in testis GSH, GPx, CAT, SOD, and GST activity and significant increase (P<0.05) in MDA concentration compared with the untreated control animals. TEO pre-treatment protected testis from the H2O2 generated oxidative stress. These results were confirmed by histological architecture examinations.
CONCLUSIONH2O2 has the ability to alter the sperm function, characteristics and development of testis. However, TEO is an efficient natural agent, which can prevent the testis from H2O2-induced oxidative damage in rats.