Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China.

Wei Liu; Hui Xin Liu; Lin Zhang; Xue Xia Hou; Kang Lin Wan; Qin Hao

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Evaluation of Six Recombinant Proteins for Serological Diagnosis of Lyme Borreliosis in China.

Author: Wei LIU ^{1
,

2} ; Hui Xin LIU ¹ ; Lin ZHANG ¹ ; Xue Xia HOU ¹ ; Kang Lin WAN ¹ ; Qin HAO ¹ ;
Author Information

1. State Key Laboratory for Infectious Diseases Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 102206, China
2. Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou 310003, Zhejiang, China.
Publication Type:Journal Article
Keywords: Logistic regression; Lyme borreliosis; ROC; Recombinant proteins; Serological diagnosis
MeSH: Antigens, Bacterial; blood; Bacterial Proteins; analysis; China; Enzyme-Linked Immunosorbent Assay; veterinary; Lyme Disease; diagnosis; Recombinant Proteins; analysis; Sensitivity and Specificity; Serologic Tests; veterinary
From: Biomedical and Environmental Sciences 2016;29(5):323-330
CountryChina
Language:English
Abstract:
OBJECTIVEIn this study, we evaluated the diagnostic efficiency of six recombinant proteins for the serodiagnosis of Lyme borreliosis (LB) and screened out the appropriate antigens to support the production of a Chinese clinical ELISA (enzyme-linked immunosorbent assay) kit for LB.
METHODSSix recombinant antigens, Fla B.g, OspC B.a, OspC B.g, P39 B.g, P83 B.g, and VlsE B.a, were used for ELISA to detect serum antibodies in LB, syphilis, and healthy controls. The ELISA results were used to generate receiver operating characteristic (ROC) curves, and the sensitivity and specificity of each protein was evaluated. All recombinant proteins were evaluated and screened by using logistic regression models.
RESULTSTwo IgG (VlsE and OspC B.g) and two IgM (OspC B.g and OspC B.a) antigens were left by the logistic regression model screened. VlsE had the highest specificity for syphilis samples in the IgG test (87.7%, P<0.05). OspC B.g had the highest diagnostic value in the IgM test (AUC=0.871). Interactive effects between OspC B.a and Fla B.g could reduce the specificity of the ELISA.
CONCLUSIONThree recombinant antigens, OspC B.g, OspC B.a, and VlsE B.a, were useful for ELISAs of LB. Additionally, the interaction between OspC B.a and Fla B.g should be examined in future research.