Rapid and Sensitive Chemiluminescent Enzyme Immunoassay for the Determination of Neomycin Residues in Milk.
- Author:
Peng Jie LUO
1
;
Jian Bo ZHANG
1
;
Hua Li WANG
1
;
Xia CHEN
2
;
Nan WU
3
;
Yun Feng ZHAO
1
;
Xiao Mei WANG
3
;
Hong ZHANG
1
;
Ji Yue ZHANG
1
;
Lei ZHU
1
;
Wen Xiao JIANG
3
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Anti-Bacterial Agents; metabolism; Drug Residues; metabolism; Food Contamination; analysis; Immunoenzyme Techniques; veterinary; Limit of Detection; Luminescent Measurements; veterinary; Milk; chemistry; Neomycin; metabolism
- From: Biomedical and Environmental Sciences 2016;29(5):374-378
- CountryChina
- Language:English
- Abstract: Immunoassays greatly contribute to veterinary drug residue analysis. However, there are few reports on detecting neomycin residues by immunoassay. Here, a rapid and sensitive chemiluminescent enzyme immunoassay (CLIEA) was successfully developed for neomycin residue analysis. CLIEA demonstrated good cross-reactivity for neomycin, and the IC50 value was 2.4 ng/mL in buffer. The average recovery range was 88.5%-105.4% for spiked samples (10, 50, and 100 μg/kg), and the coefficient of variation was in the range of 7.5%-14.5%. The limit of detection of CLEIA was 9.4 μg/kg, and this method was compared with the liquid chromatography-tandem mass spectrometry method using naturally contaminated samples, producing a correlation coefficient of >0.95. We demonstrate a reliable CLIEA for the rapid screening of neomycin in milk.
