Gene Frequency of Human Neutrophil Alloantigen-3 in the Korean Population.
10.17945/kjbt.2015.26.2.185
- Author:
Tae Hee HAN
1
;
Kyou Sup HAN
Author Information
1. Department of Laboratory Medicine, Inje University Sanggye Paik Hospital, Seoul, Korea.
- Publication Type:Original Article
- Keywords:
Human Neutrophil Alloantigen-3 (HNA-3);
Gene frequency;
Alloimmunization
- MeSH:
Antibodies;
Gene Frequency*;
Granulocytes;
Humans*;
Neutrophils*;
Polymerase Chain Reaction;
Pregnancy;
Tissue Donors
- From:Korean Journal of Blood Transfusion
2015;26(2):185-192
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
BACKGROUND: In the Korean population, gene frequencies of Human Neutrophil Alloantigen-1 (HNA-1), HNA-2, HNA-4, and HNA-5 were determined using serological and genotyping methods. However, no study assessing the gene frequencies of HNA-3 among the Korean population has been conducted. The aims of this study were to report the gene frequencies of HNA-3, to estimate the risk of HNA-3a (or HNA-3b) alloimmunization, and to secure donors of granulocytes for anti-HNA-3 antibodies among the Korean population. METHODS: Genotyping of HNA-3a and HNA-3b genes of 110 healthy and unrelated Korean donors was performed using a polymerase chain reaction with sequence-specific primers. RESULTS: The observed frequencies of HNA-3a and HNA-3b were 0.695 and 0.305, respectively, among the Korean population. The HNA-3a gene frequency of Koreans was significantly lower than those of American Whites and Germans (P<0.05). The risk of HNA-3a and HNA-3b alloimmunization due to pregnancy (transfusion) was 0.065 (0.084) and 0.147 (0.250), respectively, among the Korean population. The risk of HNA-3a alloimmunization was significantly higher in the Korean population than in the German and American White populations (P<0.05). CONCLUSION: The gene frequencies of HNA-3 and the risk of HNA-3a (HNA-3b) alloimmunization due to pregnancy or transfusion among the Korean population were determined. We also identified individuals who were HNA-3a/3a or HNA-3b/3b for the granulocyte panel which could be used for anti-HNA antibody identification.