Involvement of ATP-sensitive potassium channels in proliferation and differentiation of rat preadipocytes.
- Author:
Yao-Hui WANG
1
;
Hai-Yan ZHENG
;
Na-Lin QIN
;
Shang-Bin YU
;
Sheng-Yuan LIU
Author Information
1. Department of Pathophysiology, Huazhong University of Science and Technology, Wuhan 430030, China.
- Publication Type:Journal Article
- MeSH:
ATP-Binding Cassette Transporters;
genetics;
metabolism;
Adipocytes;
cytology;
Animals;
Cell Differentiation;
physiology;
Cell Proliferation;
Cells, Cultured;
KATP Channels;
physiology;
Male;
Obesity;
pathology;
PPAR gamma;
metabolism;
Potassium Channels, Inwardly Rectifying;
genetics;
metabolism;
RNA, Messenger;
genetics;
metabolism;
Rats;
Rats, Sprague-Dawley;
Receptors, Drug;
genetics;
metabolism;
Sulfonylurea Receptors
- From:
Acta Physiologica Sinica
2007;59(1):8-12
- CountryChina
- Language:English
-
Abstract:
This paper was aimed to investigate the effects of ATP-sensitive potassium channels on the proliferation and differentiation of rat preadipocytes. We examined the expression of sulphonylurea receptor 2 (SUR2) mRNA in preadipocytes and adipocytes obtained by inducing for 5 d and the effects of the inhibitor (glibenclamide) and opener (diazoxide) of ATP-sensitive potassium channels on the expression of SUR2 mRNA in preadipocytes by real-time PCR. Preadipocyte proliferation and cell cycle were measured by MTT spectrophotometry and flow cytometer. The content of intracellular lipid was measured by oil red O staining, cell diameter was determined by Image-Pro Plus 5.0 software and the expression of peroxisome proliferator-activated receptor-gamma (PPAR-gamma) mRNA was estimated by RT-PCR. SUR2 mRNA was expressed in both preadipocytes and adipocytes obtained by inducing for 5 d, and the expression in adipocytes was obviously higher than that in preadipocytes. Glibenclamide inhibited the expression of SUR2 mRNA in preadipocyte, promoted preadipocyte proliferation in a dose-dependent manner, increased the cell percentages in G(2)/M + S phase, increased lipid content, augmented adipocyte diameter, and promoted the expression of PPAR-gamma mRNA. But the actions of diazoxide were contrary to those of glibenclamide. These results suggest that ATP-sensitive potassium channels regulate the proliferation and differentiation of preadipocytes, and PPAR-gamma is probably involved in the effect of ATP-sensitive potassium channels.
