Relationship between hydrogen sulfide and myocardial damage in endotoxemic rats.
- Author:
Xiao-Hui XIAN
1
;
Xin-Li HUANG
;
Xiao-Hong ZHOU
;
Jing-Kun ZHANG
;
Yi-Ling LING
Author Information
1. Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Blood Pressure;
Endotoxemia;
metabolism;
pathology;
Hydrogen Sulfide;
blood;
Lipopolysaccharides;
toxicity;
Male;
Myocardium;
chemistry;
pathology;
Peroxidase;
metabolism;
Rats;
Rats, Wistar;
Tumor Necrosis Factor-alpha;
blood
- From:
Acta Physiologica Sinica
2007;59(3):357-362
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the changes and role of hydrogen sulfide (H2S) in myocardial damage in endotoxemic rats, a rat model of endotoxemia induced by injection of lipopolysaccharide (LPS) was developed. Male Wistar rats were divided into four groups: control group, LPS group, LPS + propargylglycine (PPG, a metabolic enzyme inhibitor of H2S) group and LPS + NaHS (H2S donor) group. The mean arterial pressure (MAP) of rats within 4 h was observed, TNF-alpha and H2S contents in plasma, TNF-alpha and H2S contents, lactate dehydrogenase (LDH) and myeloperoxidase (MPO) activity in cardiac muscles were determined. The morphological structure of cardiac muscle was observed. Administration of LPS caused a sustained fall in MAP within 4 h, and significant increases in TNF-alpha and H2S contents in plasma (P<0.05). Plasmic H2S content was negatively correlated with MAP (r = -0.936, -0.913 and -0.908 at 1, 2 and 4 h, respectively, P<0.05). LPS also induced increases in TNF-alpha and H2S contents, LDH and MPO activity in cardiac muscles and myocardial damage. Treatment with PPG reduced the increases in TNF-alpha and H2S contents in plasma, TNF-alpha and H2S contents, LDH and MPO activity in cardiac muscles, ameliorated the hypotensive effect and myocardial damage caused by LPS administration (P<0.05). However, treatment with NaHS increased TNF-alpha and H2S contents in plasma, TNF-alpha and H2S contents, LDH and MPO activity in cardiac muscles, and aggravated the hypotensive action and tissue injuries caused by LPS administration (P<0.05). It is suggested that hypotension and myocardial damage in endotoxemic rats are partly induced by increase in H2S content.
