Attenuation of streptomycin ototoxicity by tetramethylpyrazine and its effect on K⁺ channels in the outer hair cells of guinea pig cochlea.
- Author:
Hao TANG
1
;
Gui-Ying CUI
;
Li-Juan SHI
;
Qing-Hua GAO
;
Yu CAO
Author Information
1. Department of Physiology, School of Basic Medical Sciences, China Medical University, Shenyang 110001, China. tanghaocn2003@yahoo.com.cn
- Publication Type:Journal Article
- MeSH:
Animals;
Auditory Threshold;
Cochlea;
cytology;
Evoked Potentials, Auditory, Brain Stem;
Guinea Pigs;
Hair Cells, Auditory, Outer;
drug effects;
Patch-Clamp Techniques;
Potassium Channels;
metabolism;
Pyrazines;
Streptomycin;
toxicity
- From:
Acta Physiologica Sinica
2007;59(4):534-538
- CountryChina
- Language:English
-
Abstract:
In order to elucidate the mechanism underlying the attenuation of streptomycin ototoxicity by tetramethylpyrazine (TMP), the present study investigated the effect of TMP on the outward K(+) current in the outer hair cells of guinea pig cochlea. Sixty guinea pigs were divided into 6 groups randomly. Auditory brainstem response (ABR) was used to observe the change in thresholds and to evaluate ototoxicity induced by streptomycin. Whole-cell patch-clamp technique was used to observe the effect of TMP on outward K(+) current in isolated outer hair cells. The results showed that TMP attenuated the threshold shift caused by streptomycin and increased the amplitudes of Ca(2+)-sensitive K(+) current [I(K(Ca))] in the outer hair cells. The present data suggest that TMP displays anti-ototoxicity induced by streptomycin. The augmented amplitudes of I(K(Ca)) of the outer hair cells induced by TMP may be one of the mechanisms underlying its ototoxicity-attenuating effect.
