Molecular authentication of Jinyinhua formula granule by using allele-specific PCR.
10.19540/j.cnki.cjcmm.20170527.002
- Author:
Chao JIANG
1
;
Li-Chan TU
1
;
Yuan YUAN
1
;
Lu-Qi HUANG
1
;
Wei GAO
2
;
Yan JIN
1
Author Information
1. State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
2. School of Traditional Chinese Medicine, Capital Medical University, Beijing 100069, China.
- Publication Type:Journal Article
- Keywords:
allele-specific PCR;
formula granule;
molecular authentication;
Lonicerae Japonicae Flos
- From:
China Journal of Chinese Materia Medica
2017;42(13):2484-2491
- CountryChina
- Language:Chinese
-
Abstract:
Traditional authentication method is hard to identify herb's authenticity of traditional Chinese medicine(TCM) formula granules because they have lost all their morphological characteristics. In this study, a new allele-specific PCR method was established for identifying the authentication of Jinyinhua formula granule (made from Lonicerae Japonicae Flos) based on an SNP site in trnL-trnF fragment. Genomic DNA was successfully extracted from Lonicerae Japonicae Flos and its formula granules by using an improved spin column method and then PCR was performed with the designed primer. Approximately 110 bp specific bands was obtained only in the authentic Lonicerae Japonicae Flos and its formula granules, while no bands were found in fake mixed products. In addition, the PCR product sequence was proved from Lonicerae Japonicae Flos trnL-trnF sequence by using BLAST method. Therefore, DNA molecular authentication method could make up the limitations of character identification method and microscopic identification, and quickly identify herb's authenticity of TCM formula granules, with enormous potential for market supervision and quality control.
