Studies on chemical constituents of Clinopodium chinense.
10.19540/j.cnki.cjcmm.2017.0116
- Author:
Ling-Tian WANG
1
;
Zhong-Hao SUN
2
;
Ming-Liang ZHONG
2
;
Hai-Feng WU
2
;
Hai-Jing ZHANG
2
;
Nai-Liang ZHU
2
;
Gui-Bo SUN
2
;
Xiao-Xia YE
1
;
Xu-Dong XU
2
;
Yin-di ZHU
1
;
Jun-Shan YANG
2
Author Information
1. School of Pharmacy, Wenzhou Medical University, Wenzhou 325035, China.
2. Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education, Institute of Medicinal Plant Development, Chinese Academy of Medical Science & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- Keywords:
Clinopodium;
Clinopodium chinense;
favonoid;
myocardial protection;
Lamiaceae
- From:
China Journal of Chinese Materia Medica
2017;42(13):2510-2517
- CountryChina
- Language:Chinese
-
Abstract:
Twenty-eight compounds were isolated and purified from Clinopodium chinense by Sephedax LH-20, ODS, MCI and preparative HPLC. Their structures were identified as apigenin (1), apigenin-7-O-β-D-glucopyranoside (2), apigenin-7-O-β-D-glucuronopyranoside (3), thellungianol (4), apigenin-7-O-β-D-rutinoside (5), luteolin (6), luteolin-4'-O-β-D-glucopyranoside (7), apigenin-7-O-β-D-pyranglycuronate butyl ester (8), luteolin-7-O-β-D-rutinoside (9), luteolin-7-O-β-D-noehesperidoside (10), acacetin (11), acacetin-7-O-β-D-glucuronopyranoside (12), buddleoside (13), naringenin (14), pruning (15), nairutin (16), isosakuranetin (17), isosakuranin (18), didymin (19), hesperidin (20), kaempferol (21), quercetin (22), kaempferol-3-O-α-L-rahmnoside (23), p-hydroxycinnamic acid (24), caffeic acid (25), cis-3-[2-[1-(3,4-dihydroxy-phenyl)-1 -hydroxymethyl]-1,3-ben-zodioxol-5-yl]-(E)-2-propenoic acid (26), mesaconic acid (27), gentisic acid 5-O-β-D-(6'-salicylyl)-glucopyranoside (28). Among them, compounds 7, 9-10, 12, 23, 26-28 were isolated from the Clinopodium for the first time. The protective effects of compounds 1-6, 8-17 and 19 against H2O2-induced H9c2 cardiomyocyte injury were tested, compounds 15 exhibited significantly protective effects. Compared with the cell viability of (62.12±6.18)% in the model, pruning exhibited viabilities of (84.25±7.36)% at 25.0 mg•L⁻¹, respectively, using quercetin as a positive control [cell viability of (84.55±8.26)%, 20 mg•L⁻¹].
