Simultaneous determination of eight hydroxyl naphthoquinones in different parts of Arnebiae euchroma by HPLC.
10.19540/j.cnki.cjcmm.20170419.001
- Author:
Ke ZAN
1
;
Huang-Ya ZHENG
2
;
Jie LIU
1
;
Li-Nong GUO
1
;
Jian ZHENG
1
;
Shuang-Cheng MA
1
Author Information
1. National Institutes for Food and Drug Control, Beijing 100050, China.
2. Zunyi Institute for Food and Drug Control, Zunyi 730070, China.
- Publication Type:Journal Article
- Keywords:
Arnebia euchroma;
HPLC;
content determination;
different parts;
hydroxyl naphthoquinones;
isovalerylshikonin;
β, β′-dimethylacrylshikonin;
β-hydroxy-isovalerylshikonin
- From:
China Journal of Chinese Materia Medica
2017;42(13):2532-2537
- CountryChina
- Language:Chinese
-
Abstract:
This present study is to develop an HPLC method for simultaneous determination of eight hydroxyl naphthoquinones, shikonin, β-hydroxy-isovalerylshikonin, acetylshikonin, β-acetoxy-isovalerylshikonin, deoxyshikonin, isobutyrylshikonin, β,β'-dimethylacrylshikonin and isovalerylshikonin. The eight constituents were measured on a Waters Xbridge C18 column (4.6 mm×250 mm，5 μm) with isocratic elution of acetonitrile-0.05% formic acid solution (70∶30) at a flow rate of 1.0 mL•min⁻¹. The detection wavelength was 275 nm and the column temperature was 30 ℃. The results of content determination indicated that significant differences of the eight compounds exist in every part of Arnebia euchroma，in which the highest part was the root bark, followed with the root, then the stem residues. The content of the xylem of root and aerial part was lower than the above parts. The results provided scientific basis for the medicinal parts of A. euchroma.
