A new method on investigate chemical constituents which have anti-thrombin effect by HPLC.

Author: Jia-Yu WANG ¹ ; Wen-Zhen HOU ¹ ; Ya-Jie WEI ¹ ; Shan-Shan GAO ¹ ; Guang-Can BAI ¹ ; An-Dong WANG ¹ ; Chang-Hua MA ¹
Author Information

1. Institute of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China.
Publication Type:Journal Article
Keywords: Chromozym TH; HPLC; salvianolic acid; semi-micro extraction; thrombin
From: China Journal of Chinese Materia Medica 2016;41(15):2855-2860
CountryChina
Language:Chinese
Abstract: An in vitro anti-thrombin bioassay was developed to investigate the chemical constituents which have anti-thrombin effect from the water soluble components of Salvia miltiorrhiza. Using Chromozym TH as a probe combined with ethyl acetate Semi-micro extraction was applied to measure p-nitroaniline by HPLC. According to the results, the inactivationrate of thrombin by sodium danshensu, salvianolic acid A and salvianolic acid B under a given set of conditions were 3.06%, 77.77% and 2.35%, respectively. In the water-soluble components, salvianolic acid A has a direct inhibition of thrombin, while sodium danshensu and salvianolic acid B have no significant effect on thrombin. The method is sensitive and low consumption. It can eliminate the interference absorbed for the sample itself which can be used for screening single or multiple direct antithrombin active ingredient of herbal extract.