Isolation and identification of berberine from endophytic fungi HL-Y-3.

Author: Feng-Hua ZHANG ¹ ; Jian-Hui XIANG ¹ ; Wen-Xia CUI ¹ ; Jiang YU ¹ ; Yan WANG ¹ ; Qin-Fan LI ¹
Author Information

1. College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.
Publication Type:Journal Article
Keywords: Coptis chinensis; berberine; endophytic fungi; identification
From: China Journal of Chinese Materia Medica 2016;41(16):2998-3001
CountryChina
Language:Chinese
Abstract: The endophytic fungi HL-Y-3, which was isolated from the healthy leaves of Coptis chinensis, produced berberine when grown in the PDA culture medium. The presence of berberine was confirmed by the chromatographic and spectroscopic analyses. The yield of berberine was recorded as 9.313 μg•g⁻¹ by HPLC. The strain HL-Y-3 was identified as Alternaria sp.by morphological observation and 5.8S rDNA-ITS sequence analysis.The separation and purification of constituents were performed by PTLC. The mass spectrometry (MS) of the analyte was shown to be identical with authentic berberine.Further analysis with nuclear magnetic resonance (NMR) spectroscopy to showed that the chemical structure of the fungal berberine was identical with authentic berberine. The research provided new resources for the utilization of berberine.