Caudal-related homeobox 2 gene expression and its effects on human gastric carcinoma cell line.
- Author:
Lei LI
1
;
Qiang XIAO
;
Yu-bo XIE
;
Yu-lin MA
;
Zhen-yong TANG
;
Yong-shuo YIN
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Cycle; Cell Line, Tumor; Genetic Vectors; Homeodomain Proteins; genetics; metabolism; Humans; Plasmids; genetics; RNA, Messenger; genetics; Stomach Neoplasms; metabolism; pathology; Transfection
- From: Chinese Journal of Surgery 2008;46(11):847-850
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of human caudal-related homeobox 2 (Cdx2) gene expression on human gastric carcinoma cell line MGC-803.
METHODSpCMV-Cdx2-HA eukaryotic expression plasmid was constructed by using DNA recombinant method. The MGC-803 cells were divided into 4 groups: non-transfected, transfected with pCMV-HA, transfected with pCMV-GAPDH-HA, transfected with pCMV-Cdx2-HA. Cdx2 gene and its protein expression was detected by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot techniques respectively. The effects of Cdx2 overexpression on the growth of MGC-803 cells in vitro was assessed by measuring MTT, flow cytometry and transmission electron microscopy analysis.
RESULTSIn MGC-803 cells transfected with pCMV-Cdx2-HA, cell proliferation was significantly suppressed, the cells was ultrastructurally destroyed, cell cycle progression was blocked in G0/G1 and apoptosis rate was higher (P<0.05) in comparison with non-transfected cells or cells transfected with empty vector.
CONCLUSIONIt indicated that Cdx2 gene can suppress the growth of gastric carcinoma and may save as a novel therapeutic target.
