The clinical significance of heparanase expression in human pancreatic carcinoma tissue.
- Author:
Jiong CHEN
1
;
Chun LEI
;
Min DU
;
Wen-bo LI
;
Hou-kuo TANG
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Female; Glucuronidase; biosynthesis; genetics; Humans; Male; Middle Aged; Pancreatic Neoplasms; enzymology; pathology; RNA, Messenger; genetics
- From: Chinese Journal of Surgery 2008;46(19):1502-1504
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between heparanase 1 (Hpa1) expression and the development of human pancreatic cancer.
METHODSReal-time PCR and Western blot methods were used the expression of Hpa1 in 37 cases of pancreatic cancer were analyzed quantitatively. The relationship between the expression of Hpa1 and the clinicopathological features of pancreatic cancer was analyzed.
RESULTSThe expression of Hpa1 mRNA in pancreatic cancer was 23.53 +/- 4.13, in pancreatic tissues was 4.08 +/- 2.14 and in pancreatic cancer adjacent tissue was 16.93 +/- 3.06 by real-time PCR detected (P < 0.01). The expression of Hpa1 protein in normal tissue was 0.36 +/- 0.14, in pancreatic cancer adjacent tissue was 1.21 +/- 0.37 and in pancreatic cancer tissue was 1.76 +/- 0.28 by Western blot detected (P < 0.05). The expression of Hpa1 mRNA and Hpa1 protein in pancreatic cancer tissue were both higher than pancreatic cancer adjacent tissue and normal tissue. The expression of Hpa1 was significant associated with TNM grade and Invasion to nerves or vascellum lymph node metastasis separately (P < 0.05). But no relationship between histological differentiation and the tumor size (P > 0.05).
CONCLUSIONSThe Hpa1 overexpressed in pancreatic cancer tissues and its abnormal expression may be involved in the oncogenesis and development of pancreatic cancer.
