Morphological study on the role of ICC-like cells in detrusor neuro-modulation of rat urinary bladder.
- Author:
Qiang FANG
1
;
Jing YANG
;
Jin-hong PAN
;
Wei-bing LI
;
Wen-hao SHEN
;
Long-kun LI
;
Yong-quan WANG
;
Jian-li FENG
;
Li-li DING
;
Bo SONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Female; Gap Junctions; Guinea Pigs; Male; Muscle, Smooth; innervation; Myocytes, Smooth Muscle; cytology; ultrastructure; Nerve Endings; ultrastructure; Rats; Rats, Sprague-Dawley; Urinary Bladder; cytology; innervation; ultrastructure
- From: Chinese Journal of Surgery 2008;46(20):1542-1545
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the role of ICC-like cells in bladder neuromodulation in rat urinary bladder.
METHODS14 SD rats and 1 guinea pig were sacrificed in this study. The ultra structural relationships among interstitial cells, nerves and detrusor smooth muscle cells (DSMCs) of urinary bladder were investigated by transmission electron microscopy (TEM). c-kit immunofluorescence was used to identify ICC-like cells in SD rat urinary bladder and the structural relationship between ICC-like cells and nerve terminals was studied by immunofluorescence (double-label).
RESULTSGap junction between ICC-like cells and DSMCs was confirmed by TEM. ICC-like cells were very close apposition with nerve terminals under TEM. ICC-like cells were identified to exist in sub-urothelium layer, along the longitude of smooth muscle bundles and among detrusor smooth muscle in SD rat urinary bladder by c-kit immunofluorescence. Double-labeled tissue with c-kit and PGP9.5 antibodies also showed that ICC-like cells were very close apposition with nerve terminals in SD rat bladder.
CONCLUSIONSMorphological study indicated that ICC-like cells in rat urinary bladder may play an important role in detrusor neuromodulation. Further study on function will be helpful for elucidating the mechanism of bladder neuromodulation clearly.
