Neural stem cells transplantation promote the expressions of brain derived neurotrophic factor after the spinal cord injury of rats.
- Author:
Yan-feng WANG
1
;
Gang LÜ
;
Yu ZHAO
;
Zhe JIN
;
Tao HUANG
;
De-shui YU
;
Bao-tie DONG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain-Derived Neurotrophic Factor; genetics; metabolism; Cells, Cultured; Disease Models, Animal; Gene Expression; Humans; Male; Mesenchymal Stem Cell Transplantation; Neurons; metabolism; transplantation; Random Allocation; Rats; Rats, Wistar; Spinal Cord Injuries; genetics; metabolism; surgery; therapy; Up-Regulation
- From: China Journal of Orthopaedics and Traumatology 2008;21(11):836-838
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of neural stem cells (NSCs) transplantation on the brain derived neurotrophic factor (BDNF) after the spinal cord injury (SCI) of rats, and to investigate the mechanism of repairing the SCI by NSCs transplantation.
METHODSNeural stem cells were cultured from the hippocampus of rats' embryo and identified by immunocytochemistry. Seven days after the operation of SCI, the NSCs were transplanted into the injured site. Sixty adult Wistar rats were randomly divided into three groups: SCI cured with NSCs transplantation (group A), SCI received DMEM solution (group B), control group (group C). Then the expression of BDNF of the lesion and neighbor areas were examined by reverse transcsription polymerase chain reaction (RT-PCR) and immunohistochemistry, so as to investigated the mechanism of repairing the SCI after NSCS transplantation.
RESULTSAccording the RT-PCR results analysis, the expression of BDNF mRNA of group A enhanced higher than that of group B on the 1st, 3rd, 5th day after transplantation of NSCs. According the immunohistochemistry results analysis, the expression of BDNF mRNA of group A enhanced higher than that of group B on the 7th, 14th, 28th day similarly.
CONCLUSIONThe transplantation of NSCs can change the tiny-entironment by upregulating the expression of BDNF. It maybe one of the mechanism of repairing the SCI by NSCs transplantation.
